16th November 2021 | 10:00am EST | Marina Kirkitadze, Head of Process Support & PAT Platform, Analytical Sciences at Sanofi Pasteur and Gabriella Gerzon, PhD Candidate, York University, Toronto, Canada and Dean Roberts, Director of Market and Technology Development, Bruker Applied Spectroscopy, North America |WATCH FOR FREE
Near infrared Spectroscopy (NIR) is a tool that has been continuously developed and used in PAT for bioprocess monitoring. Therefore, we evaluated the feasibility of using near-infrared spectroscopy (NIR) as an analytical method to monitor in-line process parameters for C. tetani bioprocess monitoring and the Maillard reaction. Critical process parameters were evaluated from C. tetani bioprocess samples from various fermentations, and multivariate models were created and compared between off-line and in-line measurements. Furthermore, in-line analysis was further tested to evaluate variation in heat-treated media to assess Maillard browning. Therefore, the results of the study help highlight a potential application of using NIR to evaluate critical process parameters involved in the C. tetani bioprocess and Maillard browning.
Presented by Marina Kirkitadze, Head of Process Support & PAT Platform, Analytical Sciences at Sanofi Pasteur
Marina Kirkitadze is Head of Process Support and PAT Platform, Analytical Sciences North America, Sanofi Pasteur Ltd., Toronto Canada. She has 19 years of experience in the vaccine industry. Marina received her PhD in Biological Sciences at the Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia, and her MBA from the University of Phoenix, AZ, USA. Prior to joining Sanofi Pasteur, Marina was a postdoctoral fellow at the Department of Chemistry, University of Edinburgh, and then at the Center for Neurological Diseases, Brigham and Women’s Hospital and Harvard Medical School. Marina’s focus is on the development and implementation of PAT solutions for in-line monitoring of media components, protein concentration and conformation, process-related residuals, and protein adsorption. Her contributions went towards the CMC section in eBLA application for Adacel, approvals for the new manufacturing facility and its extension, Pentacel, and to Module 3 CTD of Hexaxim vaccine submission. Marina has published 40 manuscripts.
Followed by Gabriella Gerzon, PhD Candidate, York University, Toronto, Canada
Gabriella Gerzon is currently a PhD student in the Biology Department at York University., Toronto, Canada. Gabriella received her MSc. in Biological Sciences from York University and her studies focused on mechanisms of epigenetic signaling in response to DNA damage. Currently, Gabriella’s research is in collaboration with Sanofi Pasteur Ltd. and focuses on the development of various analytical techniques to implement PAT solutions for bioprocess monitoring.
Followed by Dean Roberts, Director of Market and Technology Development, Bruker Applied Spectroscopy, North America
Dean Roberts is currently the Director of Market Development with Bruker Optics -Applied Spectroscopy. Dean earned a BS in Chemistry from the University of Wisconsin – Madison in 1983, specializing in Analytical Chemistry. Actively involved in Vibrational Spectroscopy, including Infrared, Near-Infrared, and Raman spectroscopy over the last 30 years, Dean has worked as an Application Scientist, Product Manager, and Field Sales Representative, first with Mattson Instruments, and then with Bruker Optics. His current role as Director of Market Development with Bruker includes identification and development of new applications for laboratory and on-line measurement of food, dairy, and agricultural products, and in development of Bruker’s Chemical and Pharmaceutical markets in North America.
Sponsored by Bruker Corporation
Bruker is a performance leader in life science and analytical systems. For over 50 years, Bruker has been driven by a single idea: to provide the best technological solution for each analytical task. Our trusted solutions encompass a wide number of analytical techniques ranging from Magnetic Resonance to Mass Spectrometry and Gas Chromatography, to Microanalysis, Optical and X-Ray Spectroscopy. These market and technology leading products are driving and facilitating many key application areas such as life science research, pharmaceutical analysis, applied analytical chemistry applications, materials research, and nanotechnology, clinical research, molecular diagnostics and homeland defense.