Why Transfection Complex Size Could Make or Break AAV Manufacturing

• Exploring the Impact of Transfection Complex Size on Productivity and Product Quality in AAV Manufacturing.
• Reviewing Benefits and Limitations of Current Methods for Controlling Transfection Complex Size.
• Implementing Scale-Independent Transfection through Strategic Control of Complex Assembly.
• Enhancing Process Robustness through Automation and Process Analytical Tools.

Presented by Vincent Wiegmann, Upstream Process Development Senior Scientist at MeiraGTx

Vincent holds an EngD in Biochemical Engineering from University College London, where their research focused on developing bioreactor scale-down models for cell culture applications. Currently, they are a Senior Scientist in the MSAT department at MeiraGTx, specializing in the development of upstream processes for AAV production. Their work emphasizes optimizing the transfection step to enhance the efficiency and scalability of gene therapy manufacturing.